Characterization of pyridoxine auxotrophs of Escherichia coli: P1 transduction.
نویسنده
چکیده
Pyridoxine mutants of Escherichia coli B, previously divided into a minimum of six groups by cross-feeding tests, were characterized by transduction studies performed with phage P1bt. The results of these studies allowed division of pyridoxine mutants into five unlinked groups and set the minimum number of enzymes between pyridoxal phosphate and a metabolite common to other pathways at six or seven, with the probable maximum at ten. One group was shown to be linked to thr, leu, and pyrA.
منابع مشابه
Isolation and characterization of pyridoxine auxotrophs of Escherichia coli.
Dempsey, Walter B. (University of Florida, Gainesville), and P. F. Pachler. Isolation and characterization of pyridoxine auxotrophs of Escherichia coli. J. Bacteriol. 91:642-645. 1966.-Pyridoxine auxotrophs of Escherichia coli B have been consistently produced by a modified penicillin enrichment method. This modified penicillin technique included a 6-hr growth period in the absence of any pyrid...
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Dempsey, Walter B. (University of Florida, Gainesville). Control of pyridoxine biosynthesis in Escherichia coli J. Bacteriol. 90:431-437. 1965.-The total pyridoxine in a culture of exponentially growing Escherichia coli was 3.6 x 10(-10) moles per mg of dry cells. One-fourth of this total was present in the medium, and was at least 90% pyridoxal 5'-phosphate. Both pyridoxol and pyridoxal, when ...
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Nutritional auxotrophs of Escherichia coli synthesize vitamin B(6) compounds at a rate of 1 x 10(-10) to 2 x 10(-10) moles per hr per mg (dry weight) of cells when they are suspended in minimal medium lacking their required nutrients. A few auxotrophs have been found to stop or reduce vitamin B(6) synthesis during such an experiment. These include thiamineless, citrate synthaseless, and pyridox...
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عنوان ژورنال:
- Journal of bacteriology
دوره 97 3 شماره
صفحات -
تاریخ انتشار 1969